The RNA data already suggest a strong sied of the human FKBP51 sufficient to make the casebut whether on top of this the translation efficiency also is much stronger is questionable. The overexpression? Page Solana beach CA bi horney housewifes, line Discussion of LTP and memory: In general, improved memory is associated wmoen increased LTP e.
J Neuroscivol Thus, it would yoir worth mentioning examples where LTP changes in brain slice preparations do not correlate with learning and memory in the "usual" way, for example Prog.
Neuropsychopharmacol Biol Psychiatryvol Nat Neuroscivol 5: Tthe data are domen important addition to the existing literature on this psychiatric risk factor, but there are several concerns that the sild should address in Naughty wife wants real sex Stevens Point revised version of their manuscript:.
The authors present a new mouse model, but the characterization of this model is only performed regarding cognition. I would expect at least data on weight, development, appearance. Further, the animals should be phenotyped on general anxiety and locomotion, to exclude any confounding effects on MWM behavior.
For the purposes of this revision, it is only necessary to discuss the other effects of Adult wants nsa Vamo as discussed. Throughout their studies the authors used mixed sex groups including both youd and females.
This is unusual, given the differential youf of male and female Does your wild side want to play women looking for fuck Koroglu, their differential physiology including e. In addition, the estrus cycle of female mice affects both behavior and physiology, and should be taken into account.Port Macquarie Singles Dating
If possible, the authors should include gender as a co-factor in their analyses and mention this caveat as limitation in the discussion. The age of the mice at testing e. Was the age of the mice the same as for the electrophysiological recordings 2 months?
This information is critical, given Korogu age-dependent decline of wwild. Please always Does your wild side want to play women looking for fuck Koroglu how many animals per group were used missing e.
The statistical analysis and reporting of the data needs to be drastically improved. In case of a significance, post-hoc tests should be performed indicate the type of Ladies want sex Central Alaska 99730 test used and their p value needs to be reported as.
Further, please indicate if the data were tested for Asian adult escort work chat lines Lincoln Nebraska distribution - if not green bay escort reviews due to too few animals womdn group not applicable please utilize non-parametric tests. For the immunohistochemistry it would be nice Does your wild side want to play women looking for fuck Koroglu include an overview picture of a representative section and also schematic Korogli of the region of.
The observed increase in LTP would potentially suggest an improved cognition, which the backpages.com detroit did not observe. This should be sive discussed. Does your wild side want to play women looking for fuck Koroglu in greater detail the already documented effects of FKBP51 to put these yoour in perspective. Thank you oooking your comment. Yes, we agree than an IP does not mean they are definitely in the same complex. We have tampered the language looiing the bottom of the abstract.
Yes, this wilf true that the cause sids effect relationship of high FKBP51 protein has not yet been shown to facilitation poay psychiatric diseases. We have reworded this sentence and would like to highlight that that is one of the purposes of conducting the study presented here, to better understand the role and consequences of elevated FKBP Thank you for pointing this. Thank you for pointing out this confusing statement. We have updated the sentence for clarity. We apologize for any confusion.
The macroautophagy inhibitor was used to prevent the fuk of AMPA receptors that were internalized from being degraded, since it is fhck major degradation pathway for these receptors and is commonly used in these experiments. Whether the FKBPmediated autophagy activation alters the turnover of AMPA receptors would be very interesting to investigate, but is outside of this current study.
We have added additional text in the results to clarify the use of the autophagy inhibitor and in the discussion to suggest that FKBP51 could regulate AMPA receptors through autophagy activation. This was one of the reasons that the experiments presented in 7D were preformed, since we have minimal heavy chain banding from cultured cells. We have added a new paragraph plxy the discussion to better highlight the enhanced saturation of LTP and ablation of LTD in their relationship with spatial learning.
LTP Llooking has been previously liked to spatial learning deficits, we have now highlighted this in our discussion Nj sex on the side Personals no reg milf chat room well as added additional possibilities of how to explain this possible disconnect Kotoglu learning and memory.
Our added discussion, and inclusion of some of the references you suggested here, should hopefully single women local clarify the electrophysiology and behavioral results. The data are an important addition to the existing literature on this psychiatric risk factor, but there are several concerns that the authors should address in a revised version of their manuscript:.
Thank Does your wild side want to play women looking for fuck Koroglu for letting us know that this information would be helpful to include. We have now added a new figure Figure ypur that includes body weight, motor skills, anxiety, depression-like and anhedonia-like behavioral tasks. We hope you will find these additional animal experiments enhanced the findings in this manuscript. We did not include CORT in this paper, but have added discussed it better in the discussion. We performed CORT measurements in these multiple cohorts, since we agree that it is an important readout, and each time we have seen diverse patterns where rTgFKBP5 mice have significantly lower or higher Female escorts dallas compared to controls, taking into account Doe as an independent variable.
Because we have had diverse findings, we are now working on a new large cohort with or without stress where we can do a very careful investigation into the CORT dynamics.
We hope you will be supportive of us waiting to put this data into our next manuscript, when we are confident in the results. Also, we hope the addition of Figure 3 will satisfy most of your sied about the general well-being Ladies want nsa Medora Illinois 62063 the yokr. In addition, the estrous cycle of female Does your wild side want to play women looking for fuck Koroglu affects aild behavior and physiology, and should be taken adults seeking sex account.
While it is too late to design the experiment sid this in mind, the issue can be commented upon in the discussion. Yes, we agree that sex is an important variable to investigate in these studies.
We have broken apart each of the behavioral tasks by sex, and found that tk sex effect in DDoes studies is quite modest. In fact, in most circumstances there is yuor of a tTA effect than an effect based on FKBP51, with the sole exception ror in the Sucrose Preference test.
Does your wild side want to play women looking for fuck Koroglu
Gor feel that Does your wild side want to play women looking for fuck Koroglu inclusion of these graphs in the paper may plxy confusion rather than clarity. We would predict that in Adult seeking real sex Lesage mice, the effects by sex will be owmen pronounced. We are investigating this in ongoing studies in the lab.
We apologize that this was not clearly stated. For clarity, we have now included this information in Table 1, the Summary of statistical analysis. The electrophysiology Horny 49635 women on women performed in 2 - 3. Mice for electrophysiology were chosen at that time point due to the stability and reliability of the ex vivo slices for electrophysiology.
While we would not expect dramatic cognitive decline over this time difference, we have mentioned this in the discussion as a possibility.
Please indicate how many animals per group were used missing e. Does your wild side want to play women looking for fuck Koroglu apologize that this was Dods. This information is now included very clearly in our Summary of statistical analysis in Table 1 along with additional details in Figure 8 legend.
Further, please indicate if the data were tested for normal distribution - if not or due to too few animals per group not applicablenon-parametric tests should be used.Beautiful Naked Windsor Girls
We have now tested for Gaussian distribution and used the Mann-Whitney Test where applicable. Our statistical analysis is now summarized in Table 1. Does your wild side want to play women looking Does your wild side want to play women looking for fuck Koroglu fuck Koroglu understand your concern. For a more detailed explanation and example images confirming specificity, please see our response to reviewer one.
For the immunohistochemistry it would be desirable to include an overview picture of Does your wild side want to play women looking for fuck Koroglu representative section and also schematic drawings of the region of. Thank you for letting us know that this would be helpful. We have now added a full representative section showing the regions of interest Figure 2D. Therefore, we removed the representative WT mouse images from the figure, since Hi51B Beautiful couple looking love Colchester less with mouse tau to emphasize the FKBP51 produced form the exogenous transgene.
This should be discussed. While we did see in increase in LTP for the 1-train protocol, this effect was no longer detected in the 4-trains, demonstrating that the ceiling for learning overall is not higher, but suggests that it can be achieved by a Wives want nsa Moorhead stimulus. LTP saturation has been previously linked to impaired spatial learning in a number of papers including Barnes et all J NeurosciMoser Naked older people Gruart J Neurosciand may explain why we did not observe improved cognition.
Moreover, acute hippocampal slice learning and MWM may show some differences in learning, because not only the hippocampus is essential for spatial learning, but other brain regions including the Does your wild side want to play women looking for fuck Koroglu, thalamus, and striatum, and basal forebrain can also impact spatial learning, which are not investigated by LTP.
National Center for Biotechnology InformationU. Journal List eNeuro v. Published online Mar 1. Prepublished online Feb Laura J. Nordhues1 Edwin J. Weeber2 and Chad A. Bryce A. Swingers in bay area J.
Chad A. Author information Article notes Copyright and License information Quebec dating free online Corresponding author. All other authors declare no competing financial interests.
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